BioThrust Comfycell Benchtop

Large horizontal Membrane Stirrer that creates gentle, circular flow patterns in all directions and avoids dead zones through CFD optimized flow design.

Small, high‑speed impellers that generate circulation but pronounced local shear peaks at blade tips, potentially stressing sensitive cells and causing dead zones.

Large vertical wheel that creates gentle vertical flow patterns and avoids dead zones by having a specialized bioreactor vessel. Limits transferability of process.

Static culture with gas‑permeable bottom; near‑zero hydrodynamic shear, purely diffusive with risk of gradients at higher cell densities and difficult monitoring.

Static multilayer flasks with negligible hydrodynamic shear; mass transfer is diffusion‑limited, leading to nutrient and oxygen gradients at higher cell densities.

Rocking bag with wave‑induced mixing; overall low average shear but pronounced shear peaks at the wave front and walls - complicated to scale.

Proven to be scalable from 300mL up to 200L, with up to 2000L possible depending on the desired processes' kLa requirements.

Without sparging, commercially viable cell culture of ATMPs is limited to between 2L and 10L. For classic biology, 1000s of liters are feasible with sparging.

Due to limited gas transfer, commercially viable cell culture of ATMPs is limited to 2L working volume - larger volumes claimed up to 80L require workarounds.

Event the largest G-Rex 500M requires scale-out (parallel handling of several G-Rex systems) to supply clinical programs, leading to high FTE costs.

Scales out only by adding more multilayer units, leading to high footprint, high manual handling effort, and considerably quality risks between flasks.

Available bag volumes can reach a few hundred liters, but complex rocking and aeration parameters mean that switching volumes is very time intensive.

STR-like design of consumable allows for flexible accommodation of PAT. 300mL consumables have three PG13.5 ports, 2L+ consumables have four PG13.4 ports.

STR design allows for flexible accommodation of PAT. The majority of standard probes that fit in a STR bioreactor will also work with a BioThrust ComfyCell.

Basic inline sensors only as limited ports and unique vessel geometry constrain additional probes and external analytics tools. Flowcell may be required.

PAT integration is very limited. Static devices with no embedded inline sensors, so monitoring relies almost entirely on incubator read‑outs and offline sampling.

No options for integrated inline probes within the multilayer flasks, so process monitoring is limited to incubator parameters and labor‑intensive offline sampling.

Moderate selection of sensors with limited ports and unique bag geometry limiting non-vendor options. Flexibility of PAT integration heavily depends on vendor.

Supports a wide range of perfusion strategies (In-situ, ATF, TFF) and is the optimal system for high intensity cell culture as bubble-free aeration de-risks processes.

Supports a wide range of perfusion strategies (ATF, TFF, spin filters, internal settlers) and is a well established platform for high‑intensity perfusion.

Perfusion for automation is possible via external loops or internal filters, but the severely limited gas transfer makes high cell densities unachievable regardless.

Primarily a fed‑batch system as medium exchange is performed in discrete steps rather than continuous perfusion, which restricts control over culture conditions.

Designed for static or intermittent medium exchange only since implementing continuous perfusion across multilayer surfaces is technically cumbersome.

Can support pefusion processes and achieve high cell densities comparable to BioThrust ComfyCell, but set-up is considerably more complex.