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Thank you for attending the webinar, we were overwhelmed by the interest in our session! Please find the recording of our US session below or on REPROCELL'S official youtube channel!
Scalable expansion of induced pluripotent stem cells (iPSCs) remains a key bottleneck for next-generation cell therapies, with conventional culture approaches limited in cell density, robustness, and scalability. Fedbatch processes, while widely used, struggle to maintain optimal nutrient supply and waste removal at high cell densities. Current bioreactor technology can’t scale high-intensity cell cultures without excessive foaming and shear force.
This webinar presents the BioThrust 300 mL single-use bioreactor, designed for straightforward operation in standard incubators or as a closed, standalone system. The novel "ComfyCell" bioreactor integrates a membrane-based Impeller and gas transfer concept that enables controlled fluid dynamics and low-shear mass transfer. A complete workflow at 300 mL is demonstrated, covering spheroid formation from single cells, expansion, harvest, and direct scale-up into a 2 L single-use ComfyCell bioreactor. At both volumes, BioThrust’s in-situ perfusion system and Membrane Stirrer enable significantly improved culture performance while maintaining full cell quality. Process analytics and cultivation data are presented, including world record cell densities up to 44×10E6 cells/mL at 300 mL and 11x10E6 cells/mL at 2 L.
These results illustrate how perfusion-based bioprocessing can enable robust, high-density iPSC manufacturing and support the development of scalable, commercially efficient cell therapy platforms.
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